3OBW
Crystal structure of two archaeal Pelotas reveal inter-domain structural plasticity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-01 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.394, 34.329, 90.109 |
Unit cell angles | 90.00, 103.64, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.600 |
Rwork | 0.228 |
R-free | 0.26700 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.439 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 10800 |
Completeness [%] | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 100mM imidazole pH 8.0, 1.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 100mM imidazole pH 8.0, 1.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 297K |