3OBL
Crystal structure of the potent anti-HIV cyanobacterial lectin from Oscillatoria Agardhii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-25 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9795, 0.9793, 0.9718 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.752, 47.275, 47.367 |
| Unit cell angles | 78.16, 62.99, 63.14 |
Refinement procedure
| Resolution | 22.640 - 1.200 |
| Rwork | 0.140 |
| R-free | 0.16923 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.356 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.640 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.079 | 0.434 |
| Number of reflections | 86175 | |
| <I/σ(I)> | 7.5 | 2.1 |
| Completeness [%] | 92.0 | 83.1 |
| Redundancy | 3.81 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.2 M NaH2PO4/0.8 M K2HPO4, 0.2 M Li2SO4, 0.1 M CAPS (pH 10.5), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
