3OB8
Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.939 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 140.381, 153.454, 217.166 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.300 - 2.800 |
R-factor | 0.2155 |
Rwork | 0.214 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.131 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 125.323 | 49.300 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.099 | 0.034 | 0.431 |
Total number of observations | 46167 | 193533 | |
Number of reflections | 115849 | ||
<I/σ(I)> | 24.5 | 18.9 | 1.9 |
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 11.9 | 11.8 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 25 % Polyethylen Glycol (PEG) 3350, 0.1 M BisTris pH 7.0, 0.2 M Sodium Tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |