3OAH
Structural Characterization of the Dual Glycan Binding Adeno-Associated Virus Serotype 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2009-10-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.917 |
Spacegroup name | H 3 2 |
Unit cell lengths | 263.000, 263.000, 609.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
Rwork | 0.275 |
R-free | 0.28750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AAV1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.480 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.150 | 0.607 |
Number of reflections | 119617 | |
<I/σ(I)> | 6.5 | 2.5 |
Completeness [%] | 72.3 | 50.5 |
Redundancy | 2.7 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 25mM Tris-HCl pH7.5 with 60 mM MgCl2 and 100mM NaCl, 4% PEG6000, and 25%glycerol, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |