3OAC

Mint deletion mutant of heterotetrameric geranyl pyrophosphate synthase in complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	92.842, 110.110, 111.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.280 - 2.600
Rwork	0.203
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3krf
RMSD bond length	0.019
RMSD bond angle	2.062
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.564
Number of reflections	33831
<I/σ(I)>	21.1	3.9
Completeness [%]	94.4	91.3
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	295	100mM Bis-Tris, 200mM ammonium acetate, 20% PEG 3350, pH 6.5, vapor diffusion, temperature 295K