3O9X
Structure of the E. coli antitoxin MqsA (YgiT/b3021) in complex with its gene promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 41 |
| Unit cell lengths | 60.996, 60.996, 148.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.146 - 2.100 |
| R-factor | 0.1813 |
| Rwork | 0.176 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3FMY and 3GA8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.146 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 31421 | |
| <I/σ(I)> | 29.13 | 2.39 |
| Completeness [%] | 99.5 | 93.5 |
| Redundancy | 6.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 0.2 M ammonium chloride, 20% PEG3350, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
