3O9O

Crystal Structure of GBS1074, an Esat-6 homologue from Group B Streptococcus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	P 65 2 2
Unit cell lengths	76.220, 76.220, 151.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.080 - 2.000
R-factor	0.1906
Rwork	0.189
R-free	0.21920
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.742
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	40.080
High resolution limit [Å]	2.000
Number of reflections	18255
<I/σ(I)>	34
Completeness [%]	99.7
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	291	0.75 M LiSO4, 0.6 M (NH4)2SO4, 0.1 M Na citrate, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 291K