3O9F
Crystal Structure of wild-type HIV-1 Protease in complex with kd27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.773, 57.934, 61.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.220 - 1.700 |
| R-factor | 0.17028 |
| Rwork | 0.169 |
| R-free | 0.20364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ? |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.377 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.053 | 0.025 | 0.371 |
| Number of reflections | 20045 | ||
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 96.7 | 99.1 | 79.7 |
| Redundancy | 6.6 | 6.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop, vapor diffusion | 6.2 | 295 | 126mM Phosphate buffer pH 6.2, 63mM Sodium Citrate, 24-29% Ammonium Sulfate, hanging drop, vapor diffusion, temperature 295K |
