3O8Y
Stable-5-Lipoxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | .97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.170, 202.890, 76.800 |
| Unit cell angles | 90.00, 109.56, 90.00 |
Refinement procedure
| Resolution | 40.770 - 2.389 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.714 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.770 | 2.450 |
| High resolution limit [Å] | 2.389 | 2.390 |
| Number of reflections | 62734 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 10% tacsimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
