3O8R
Visualizing ATP-dependent RNA Translocation by the NS3 Helicase from HCV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.033, 110.827, 141.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.681 - 2.304 |
R-factor | 0.2162 |
Rwork | 0.215 |
R-free | 0.26330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o8b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.148 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.048 | 0.392 |
Number of reflections | 64105 | |
<I/σ(I)> | 14.2 | 4.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% (w/v) PEG 3350, 160 mM LiSO4, 80 mM Bis-Tris, 10% (v/v) glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | LiSO4 | ||
3 | 1 | 1 | Bis-Tris | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 2 | PEG 3350 | ||
6 | 1 | 2 | LiSO4 | ||
7 | 1 | 2 | Bis-Tris | ||
8 | 1 | 2 | glycerol |