3O8L
Structure of phosphofructokinase from rabbit skeletal muscle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 163.684, 163.684, 356.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.000 - 3.200 |
| R-factor | 0.2378 |
| Rwork | 0.238 |
| R-free | 0.30220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o8o |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 47446 | |
| <I/σ(I)> | 17.8 | 2.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.2 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 16% PEG 400, 0.1 M MgSO4, 0.1 M acetate buffer pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
