3O8E
Structure of extracelllar portion of CD46 in complex with Adenovirus type 11 knob
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 108.160, 108.160, 222.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.800 - 2.840 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CKL and 3EXV |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 2.990 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.128 | 0.531 |
| Number of reflections | 33537 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 97.1 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | microseeding, pH 8.0, VAPOR DIFFUSION, temperature 298K |
