3O85
Giardia lamblia 15.5kD RNA binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-26 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.876, 40.611, 59.937 |
| Unit cell angles | 90.00, 100.93, 90.00 |
Refinement procedure
| Resolution | 32.845 - 1.806 |
| Rwork | 0.236 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zwz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 0.362 |
| Number of reflections | 23525 | |
| Completeness [%] | 93.9 | 85.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 0.2M Ammonium sulphate, 25%PEG 4000, Sodium acetate, pH 4.6, VAPOR DIFFUSION |
