3O7P
Crystal structure of the E.coli Fucose:proton symporter, FucP (N162A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.90922 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 119.180, 119.180, 99.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.302 - 3.196 |
R-factor | 0.2162 |
Rwork | 0.214 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o7q |
RMSD bond length | 0.026 |
RMSD bond angle | 1.124 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.302 | 3.310 |
High resolution limit [Å] | 3.196 | 3.200 |
Rmerge | 0.065 | 0.699 |
Number of reflections | 13809 | |
<I/σ(I)> | 20.1 | 1.9 |
Completeness [%] | 99.7 | 100 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 34% PEG 400, 0.1M cacodylate, 0.1M MgCl2, 0.05M NaF, 0.2M KCl, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |