3O7H
Crystal structure of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.799, 49.338, 51.687 |
| Unit cell angles | 113.18, 116.49, 94.26 |
Refinement procedure
| Resolution | 23.130 - 1.790 |
| R-factor | 0.22226 |
| Rwork | 0.221 |
| R-free | 0.24474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q37 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 31800 | |
| <I/σ(I)> | 27.8 | 4.7 |
| Completeness [%] | 93.8 | 75 |
| Redundancy | 1.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 22-26% PEG-8000, 0.25 M sodium acetate in pH 6.5 cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
