3O7A

Crystal structure of PHF13 in complex with H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97941
Spacegroup name	P 21 21 21
Unit cell lengths	21.232, 44.334, 60.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.730 - 1.670
R-factor	0.1851
Rwork	0.183
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o70
RMSD bond length	0.020
RMSD bond angle	1.586
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.660
Rmerge	0.032
Number of reflections	6772
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1M Tris-HCl pH 8.5; 1.5M Na-Citrate, PEG400 6%, VAPOR DIFFUSION, SITTING DROP, temperature 291K