3O75
Crystal structure of Cra transcriptional dual regulator from Pseudomonas putida in complex with fructose 1-phosphate'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.711, 120.220, 61.860 |
Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
Resolution | 14.944 - 2.300 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o74 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.876 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.050 |
Total number of observations | 79179 |
Number of reflections | 23340 |
<I/σ(I)> | 12.6 |
Completeness [%] | 99.8 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 294 | 0.1M Mes pH 6.5, 0.2M NaAc, 15 % PEG 8000, VAPOR DIFFUSION, temperature 294K |