3O70

PHD-type zinc finger of human PHD finger protein 13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 43 21 2
Unit cell lengths	58.315, 58.315, 73.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.380 - 1.850
R-factor	0.18422
Rwork	0.184
R-free	0.18763
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.215
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.6.0081)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.890
High resolution limit [Å]	1.850	4.560	1.850
Rmerge	0.054	0.035	0.496
Number of reflections	11409
<I/σ(I)>	16.7
Completeness [%]	99.9	99.1	99.9
Redundancy	15.5	13.6	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	291	20% PEG8000, 0.1M CHES pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K