3O6U
Crystal Structure of CPE2226 protein from Clostridium perfringens. Northeast Structural Genomics Consortium Target CpR195
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.435, 121.643, 95.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.675 - 2.500 |
| R-factor | 0.208 |
| Rwork | 0.204 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.357 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.677 |
| Number of reflections | 44436 | |
| <I/σ(I)> | 26.4 | 3.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under Paraffin oil | 7.5 | 291 | 3% PEG 3350, 3.46M potassium phosphate, 0.1M HEPES, pH 7.5, Microbatch under Paraffin oil, temperature 291K |
