3O6Q
The Structure of SpoIISA and SpoIISB, a Toxin - Antitoxin System
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97890, 0.97900, 0.97630 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.028, 59.465, 112.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.690 - 2.500 |
| R-factor | 0.19884 |
| Rwork | 0.196 |
| R-free | 0.25778 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0081) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.121 | 0.682 |
| Number of reflections | 16022 | |
| <I/σ(I)> | 18.9 | 2.7 |
| Completeness [%] | 90.5 | 44.4 |
| Redundancy | 11.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 21 % PEG 1500, 0.4 M MES, 0.2 M malic acid, 0.43 M Tris-HCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP |
