3O6M
Anti-Tat HIV 11H6H1 Fab' complexed with a 9-mer Tat peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93340 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.840, 69.720, 62.160 |
Unit cell angles | 90.00, 109.94, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.400 |
R-factor | 0.2146 |
Rwork | 0.211 |
R-free | 0.29031 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mlb |
RMSD bond length | 0.019 |
RMSD bond angle | 1.925 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.050 | 0.354 |
Number of reflections | 19524 | |
<I/σ(I)> | 21.79 | 3.79 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | soaking | 6.5 | 289 | 0.05M CaCl2, 0.1M Bis-Tris pH 6.5, 30% (v/v) polyethylene glycol 550 monomethyl ether, Soaking of Fab' crystals with 5mM Tat-peptide for 15 min, soaking, temperature 289K |