3O6C
Pyridoxal phosphate biosynthetic protein PdxJ from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 90.976, 90.976, 155.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.700 - 1.870 |
| R-factor | 0.1453 |
| Rwork | 0.144 |
| R-free | 0.17440 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.562 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.600 | 50.000 | 1.900 |
| High resolution limit [Å] | 1.870 | 5.070 | 1.870 |
| Rmerge | 0.101 | 0.052 | 0.851 |
| Number of reflections | 27387 | ||
| <I/σ(I)> | 10 | 6.1 | |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 18.1 | 16.1 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.056 M sodium phosphate, 1.344 di-potassium phosphate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
