3O6B

A Dual E3 Mechanism for Rub1 Ligation to Cdc53: Dcn1(P)-Cdc53(WHB) low resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	CCD
Collection date	2009-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97924
Spacegroup name	P 61
Unit cell lengths	123.914, 123.914, 192.558
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.100
R-factor	0.2578
Rwork	0.258
R-free	0.30830
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	4.250	3.210
High resolution limit [Å]	3.100	3.910	3.100
Number of reflections	30204
Completeness [%]	99.1	99.5	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	295	21% PEG 3350, 0.1M Bis-Tris-Propane, 0.2M NaF, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K