3O6A
F144Y/F258Y Double Mutant of Exo-beta-1,3-glucanase from Candida albicans at 2 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.573, 64.623, 94.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.230 - 2.000 |
| R-factor | 0.1567 |
| Rwork | 0.154 |
| R-free | 0.19966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cz1 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.230 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.430 |
| Number of reflections | 25035 | |
| <I/σ(I)> | 17.4 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 0.1M HEPES-KOH, 0.2M CaCl2, 19% PEG 8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
