3O6A

F144Y/F258Y Double Mutant of Exo-beta-1,3-glucanase from Candida albicans at 2 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2010-06-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.573, 64.623, 94.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.230 - 2.000
R-factor	0.1567
Rwork	0.154
R-free	0.19966
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cz1
RMSD bond length	0.020
RMSD bond angle	1.462
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.230	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.430
Number of reflections	25035
<I/σ(I)>	17.4	3.7
Completeness [%]	100.0	100
Redundancy	5.4	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	291	0.1M HEPES-KOH, 0.2M CaCl2, 19% PEG 8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K