3O6A
F144Y/F258Y Double Mutant of Exo-beta-1,3-glucanase from Candida albicans at 2 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2010-06-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.573, 64.623, 94.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.230 - 2.000 |
R-factor | 0.1567 |
Rwork | 0.154 |
R-free | 0.19966 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cz1 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.462 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.230 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.069 | 0.430 |
Number of reflections | 25035 | |
<I/σ(I)> | 17.4 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.4 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 0.1M HEPES-KOH, 0.2M CaCl2, 19% PEG 8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |