3O5Y
The Crystal Structure of the GAF domain of a two-component sensor histidine kinase from Bacillus halodurans to 2.45A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 96.961, 96.961, 104.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.990 - 2.450 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.339 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 5.280 | 2.450 |
| Rmerge | 0.066 | 0.047 | 0.626 |
| Number of reflections | 18880 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 99.6 | 97 | 100 |
| Redundancy | 7.9 | 7.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 3350, 0.2M Magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
