3O5T
Structure of DraG-GlnZ complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.654, 115.654, 105.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.000 - 2.090 |
| R-factor | 0.15315 |
| Rwork | 0.151 |
| R-free | 0.19108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3MHY and 3G9D |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.843 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.066 | 0.210 |
| Number of reflections | 31022 | |
| <I/σ(I)> | 13.75 | 3.9 |
| Completeness [%] | 97.7 | |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 12% PEG 8000, 0.2M Nacl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
