3O5P
Fk1 domain mutant A19T of FKBP51, crystal form IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.289, 54.302, 56.647 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| R-factor | 0.116 |
| Rwork | 0.112 |
| R-free | 0.14700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o5p |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.200 | 25.110 | 1.000 |
| High resolution limit [Å] | 0.950 | 3.000 | 0.950 |
| Rmerge | 0.044 | 0.032 | 0.273 |
| Total number of observations | 15574 | 8553 | |
| Number of reflections | 65715 | ||
| <I/σ(I)> | 21 | 16.2 | 2.7 |
| Completeness [%] | 79.6 | 99.5 | 31.4 |
| Redundancy | 4.2 | 5.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 25 % PEG3350, 0.2 M NH4OAc, 0.1 M HEPES, pH 7.5, vapor diffusion, temperature 293K |
