3O5F
Fk1 domain of FKBP51, crystal form VII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.679, 49.703, 66.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.650 |
| R-factor | 0.1989 |
| Rwork | 0.197 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o5p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.413 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.914 | 39.904 | 1.740 |
| High resolution limit [Å] | 1.650 | 5.220 | 1.650 |
| Rmerge | 0.049 | 0.018 | 0.455 |
| Total number of observations | 1626 | 7161 | |
| Number of reflections | 14796 | ||
| <I/σ(I)> | 14.2 | 34 | 1.7 |
| Completeness [%] | 99.3 | 96.5 | 98.6 |
| Redundancy | 3.5 | 3.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 30 % PEG2000MME, 0.1 M KSCN, pH 8.0, vapor diffusion, temperature 293K |
