3O5C
Cytochrome c Peroxidase BccP of Shewanella oneidensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-28 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.840, 81.220, 85.920 |
| Unit cell angles | 113.96, 102.10, 90.01 |
Refinement procedure
| Resolution | 40.500 - 1.800 |
| Rwork | 0.198 |
| R-free | 0.25695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hq6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.232 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.500 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.080 |
| Number of reflections | 106139 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 93.7 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | reduced with sodium dithionite, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
