3O5B

Crystal structure of dimeric KlHxk1 in crystal form VII with glucose bound (open state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-19
Detector	MAR555 FLAT PANEL
Wavelength(s)	0.8148
Spacegroup name	P 21 21 2
Unit cell lengths	98.140, 122.200, 92.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 1.970
R-factor	0.20511
Rwork	0.204
R-free	0.25506
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o08
RMSD bond length	0.017
RMSD bond angle	1.709
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	1.970	1.970
Number of reflections	78731
<I/σ(I)>	28.4	2.87
Completeness [%]	99.6	99.2
Redundancy	4.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	292	1microL reservoir + 1microL protein, reservoir: 18% PEG6000, 0.9M LiCl, 2mM MgCl2, 0.1M Bicine pH 9.0, protein: 10mg/ml Klhxk1, 10mM Tris pH 7.4, 1mM EDTA, 1mM DTT, 0.5mM PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 292K