3O59

DNA polymerase D large subunit DP2(1-300) from Pyrococcus horikoshii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-23
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9788, 0.9793, 0.9900
Spacegroup name	P 43 21 2
Unit cell lengths	54.042, 54.042, 173.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.330 - 2.200
R-factor	0.21246
Rwork	0.206
R-free	0.27168
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.774
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.058	0.233
Number of reflections	13924
<I/σ(I)>	77.9	14.4
Completeness [%]	99.0	92.4
Redundancy	27.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	22.5% PEG 10000, 0.1M HEPES-NaOH, 40mM guanidine-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K