3O55
Crystal structure of human FAD-linked augmenter of liver regeneration (ALR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-05 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.849, 76.566, 62.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.290 - 1.900 |
| R-factor | 0.18916 |
| Rwork | 0.182 |
| R-free | 0.26659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hj3 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.983 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | |
| Number of reflections | 9759 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 98.5 | 93 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1 M MES, 20% PEG6000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
