3O4Z
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.400, 123.300, 162.300 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 39.200 - 3.100 |
| R-factor | 0.22267 |
| Rwork | 0.222 |
| R-free | 0.23857 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 60224 | |
| <I/σ(I)> | 24.2 | 2.5 |
| Completeness [%] | 97.6 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M Tris-HCl, 7% (w/v) polyethylene glycol (PEG) 8000, 0.8 M NaCl and 1.5% (v/v) ethanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
