3O4Y
Crystal structure of CAD domain of the Plasmodium Vivax CDPK, PVX_11610
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.106, 84.858, 85.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.200 |
| R-factor | 0.2366 |
| Rwork | 0.235 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k21 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.347 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.061 | 0.035 | 0.608 |
| Number of reflections | 16607 | ||
| <I/σ(I)> | 8.3 | 3.06 | |
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 7.3 | 6.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % PEG 3350, 0.2 M NH4diHPO4, 5 mM MgCl2, 20% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
