3O4N
Crystal structure of the Rous Associated Virus Integrase catalytic domain in MES buffer pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.281060 |
| Spacegroup name | P 61 |
| Unit cell lengths | 107.500, 107.500, 50.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.680 - 1.800 |
| R-factor | 0.19074 |
| Rwork | 0.189 |
| R-free | 0.22632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vsd |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.118 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 30860 | |
| <I/σ(I)> | 11.61 | 3.44 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.25 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 290 | 10 mM ZnCl2, 20% (w/v) PEG 6000, 100 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
