Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3O4A

Crystal structure of Symfoil-2: de novo designed beta-trefoil architecture with symmetric primary structure

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyCCD
Collection date2009-09-25
DetectorADSC QUANTUM 315
Wavelength(s)0.97950
Spacegroup nameP 1 21 1
Unit cell lengths50.701, 53.641, 85.336
Unit cell angles90.00, 90.07, 90.00
Refinement procedure
Resolution45.414 - 1.450
R-factor0.1868
Rwork0.186
R-free0.21530
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006
RMSD bond angle1.090
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASES
Refinement softwarePHENIX ((phenix.refine))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.480
High resolution limit [Å]1.4501.450
Rmerge0.0830.398
Number of reflections73170
<I/σ(I)>34.12.9
Completeness [%]92.395.1
Redundancy5.12.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP72982M ammonium sulfate, 0.2M Li2SO4, 0.1M Tris, 15mg/mL protein concentration, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

222624

PDB entries from 2024-07-17

PDB statisticsPDBj update infoContact PDBjnumon