3O46
Crystal structure of the PDZ domain of MPP7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97904 |
Spacegroup name | P 63 |
Unit cell lengths | 66.616, 66.616, 46.475 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.308 - 1.300 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.19080 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.679 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.320 |
High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
Rmerge | 0.041 | 0.025 | 0.751 |
Number of reflections | 28982 | ||
<I/σ(I)> | 14.6 | ||
Completeness [%] | 99.8 | 97.7 | 100 |
Redundancy | 5.6 | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 30% PEG-1500, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 30% PEG-1500, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K |