3O40
Complex of a chimeric alpha/beta-peptide based on the gp41 CHR domain bound to gp41-5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.846, 56.968, 103.466 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.100 |
R-factor | 0.17264 |
Rwork | 0.171 |
R-free | 0.21206 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o3x |
RMSD bond length | 0.014 |
RMSD bond angle | 1.306 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 16963 |
<I/σ(I)> | 15.6 |
Completeness [%] | 99.7 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2 M MgCl2, 0.1 M Tris pH 8.5, 30% w/v PEG 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |