3O3Y
A chimeric alpha+alpha/beta peptide based on the CHR domain sequence of gp41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 33.036, 33.036, 48.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.350 |
| R-factor | 0.14401 |
| Rwork | 0.143 |
| R-free | 0.17092 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f4z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.350 |
| Number of reflections | 7108 |
| <I/σ(I)> | 44.2 |
| Completeness [%] | 99.3 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.3 M NaCl, 0.1 M Tris pH 8.5, 35% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
