3O3W
Crystal Structure of BH2092 protein (residues 14-131) from Bacillus halodurans, Northeast Structural Genomics Consortium Target BhR228A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-23 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 31 |
| Unit cell lengths | 114.239, 114.239, 85.452 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.790 - 2.910 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (FOLLOWED BY SOLVE/RESOLVE) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.166 | 0.420 |
| Number of reflections | 54786 | |
| <I/σ(I)> | 12.65 | 3.18 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M sodium acetate (pH 5), 20% PEG 400, and 0.1M KH2PO4, Microbatch, under oil, temperature 277K |
