3O3K
Crystal structure of AF1382 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.9 |
| Spacegroup name | P 42 |
| Unit cell lengths | 53.540, 53.540, 41.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.860 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26800 |
| Structure solution method | Sulfur SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SGXPRO |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 5325 | |
| <I/σ(I)> | 124.7 | 17.4 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 25 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 291 | USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (2.8 MG/ML) AND SOLUTION CONTAING 0.1M SODIUM CITRATE/CITRIC ACID PH 5.5, 0.1M SODIUM CHLORIDE, 0.1M LITHIUM SULPHATE, 30% V/V PEG 400, Microbatch, temperature 291K |
