3O3J
Crystal structure of Arabidopsis thaliana peptide deformylase 1B (AtPDF1B) in complex with inhibitor 6b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Collection date | 2008-10-15 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.500, 57.500, 144.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 3.000 |
| R-factor | 0.20902 |
| Rwork | 0.200 |
| R-free | 0.28506 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m6o |
| RMSD bond length | 0.050 |
| RMSD bond angle | 3.918 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9113 | |
| <I/σ(I)> | 13.5 | 3 |
| Completeness [%] | 98.1 | 98.1 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG550MME, ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
