3O3I
Crystal Structure of human Hiwi1 PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OH at its 3'-end
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 44.303, 44.303, 147.707 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.801 |
R-factor | 0.24494 |
Rwork | 0.243 |
R-free | 0.29701 |
Starting model (for MR) | Hiwi1(V361M)PAZ domain |
RMSD bond length | 0.005 |
RMSD bond angle | 0.849 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 5.980 | 2.800 |
Rmerge | 0.096 | 0.049 | 0.361 |
Number of reflections | 3966 | ||
<I/σ(I)> | 14.8 | 1.9 | |
Completeness [%] | 96.6 | 95.4 | 78.7 |
Redundancy | 4.1 | 3.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 1-5% PEG3350, 50 mM NaCl, 50 mM Na-acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |