3O3G
T. maritima RNase H2 in complex with nucleic acid substrate and calcium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.122, 47.347, 79.012 |
| Unit cell angles | 90.00, 132.81, 90.00 |
Refinement procedure
| Resolution | 28.750 - 2.100 |
| R-factor | 0.1747 |
| Rwork | 0.171 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o3f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.484 |
| Number of reflections | 16994 | |
| <I/σ(I)> | 20.5 | 2.8 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.3 M CaCl2, 0.1 M Tris-HCl, 20% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CaCl2 | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | PEG 3350 | ||
| 4 | 1 | 2 | CaCl2 | ||
| 5 | 1 | 2 | Tris-HCl | ||
| 6 | 1 | 2 | PEG 3350 |
