3O3F
T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.062, 48.570, 78.391 |
| Unit cell angles | 90.00, 131.80, 90.00 |
Refinement procedure
| Resolution | 29.224 - 2.000 |
| R-factor | 0.1939 |
| Rwork | 0.191 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.424 |
| Number of reflections | 19185 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 94.9 | 68.4 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.3 M MgCl2, 23% PEG 3350, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | HEPES |
