3O3C
Glycogen synthase basal state UDP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.586, 167.209, 121.251 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 48.288 - 3.512 |
| R-factor | 0.2209 |
| Rwork | 0.219 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3naz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.114 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.570 |
| High resolution limit [Å] | 3.510 | 9.510 | 3.510 |
| Rmerge | 0.110 | 0.065 | 0.444 |
| Number of reflections | 46381 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 99.0 | 99.5 | 92.7 |
| Redundancy | 3.6 | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris pH 8.0, 20% PEG 3500, 200 mM LI2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
