3O2U
S. cerevisiae Ubc12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-03-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.451, 35.938, 87.035 |
Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
Resolution | 25.921 - 2.003 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y8x Chain A |
RMSD bond length | 0.007 |
RMSD bond angle | 1.164 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 23534 |
Completeness [%] | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | 27% PEG 3350, 0.1M Tris, 0.2M NaCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |