3O2T
Crystal structure of the N-terminal domain of human Symplekin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.596, 63.033, 62.283 |
| Unit cell angles | 90.00, 90.59, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.16034 |
| Rwork | 0.159 |
| R-free | 0.19247 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.527 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (THEN SOLVE/RESOLVE) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.029 | 0.492 |
| Number of reflections | 62696 | |
| <I/σ(I)> | 40.3676 | 1.697 |
| Completeness [%] | 98.6 | 90.9 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50mM Bis-Tris, 40mM ammonium sulfate, 40% (v/v) pentaerythritol ethoxylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
