3O2O
Structure of E. coli ClpS ring complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.910, 155.870, 71.230 |
| Unit cell angles | 90.00, 114.64, 90.00 |
Refinement procedure
| Resolution | 24.967 - 2.900 |
| R-factor | 0.1754 |
| Rwork | 0.173 |
| R-free | 0.20110 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.788 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 37634 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 0.1 M Sodium Nitrate 20% PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
