3O2I
The crystal structure of a functionally unknown protein from Leptospirillum sp. Group II UBA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929, 0.97943 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.485, 91.630, 171.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.314 - 2.197 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.25080 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.079 | 0.348 |
| Number of reflections | 20399 | |
| <I/σ(I)> | 25.7 | 3.4 |
| Completeness [%] | 98.1 | 84.7 |
| Redundancy | 9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.1M MES, 20% PEG10K, 1/20 TEV, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
